{"id":4299,"date":"2021-08-16T14:17:24","date_gmt":"2021-08-16T07:17:24","guid":{"rendered":"https:\/\/2021.icsae.id\/?p=4299"},"modified":"2021-08-21T21:54:32","modified_gmt":"2021-08-21T14:54:32","slug":"icsae8-video-id148","status":"publish","type":"post","link":"https:\/\/2021.icsae.id\/icsae8-video-id148\/","title":{"rendered":"ICSAE8 Video ID148 Ficus septica, an Ecosystem Keystone Species"},"content":{"rendered":"\t\t
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Paper ID: 148<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Ficus septica<\/i>, an Ecosystem Keystone Species Induced ROS-Mediated Cytotoxicity in HepG2 Hepatocarcinoma Cells<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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S Haryanti*<\/sup>, N Rahmawati, I Y M Sholikhah and Y Widiyastuti<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Medicinal Plant and Traditional Medicine Research and Development Center, Ministry of Health, Jl. Raya Lawu No 11 Tawangmangu, Karanganyar, Indonesia<\/p>

Email: sari.haryanti@gmail.com<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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Ficus septica<\/em> grows in all around Indonesia, as one of the key species in various ecosystem types. This plant is a food resource and habitat for some animals. This study aimed to examine cytotoxic activity of F.<\/em> septica<\/em> extract in HepG2 cells. The leaves powder was macerated using ethanol 96%. Cytotoxic activity was evaluated by MTT assay. The determination of cell cycle profile and reactive oxygen species (ROS) were done by flow cytometry. The extract inhibited the growth of HepG2 and Vero cells with an IC50<\/sub> of 31 and 286 \u00b5g\/mL, while doxorubicin 0.8 and 117 \u00b5g\/mL. The selectivity index of the extract and doxorubicin was 9 and 146 respectively. The extract triggered cell cycle arrest in HepG2 at the G0\/G1 phase, whereas doxorubicin in the S phase. Both extracts and doxorubicin significantly increased intracellular ROS in HepG2, but not in normal Vero cells. In conclusion, our findings suggest that F. septica<\/em> induced cytotoxicity in HepG2 cells are mediated by oxidative stress.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Paper ID: 148 Ficus septica, an Ecosystem Keystone Species Induced ROS-Mediated Cytotoxicity in HepG2 Hepatocarcinoma Cells S Haryanti*, N Rahmawati, I Y M Sholikhah and Y Widiyastuti Author Information Medicinal Plant and Traditional Medicine Research and Development Center, Ministry of Health, Jl. Raya Lawu No 11 Tawangmangu, Karanganyar, Indonesia Email: sari.haryanti@gmail.com View Abstract Ficus septica… Read More »ICSAE8 Video ID148 Ficus septica, an Ecosystem Keystone Species<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":5079,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"off","neve_meta_content_width":70,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"on","footnotes":"","_jetpack_memberships_contains_paid_content":false},"categories":[11],"tags":[],"yoast_head":"\nICSAE8 Video ID148 Ficus septica, an Ecosystem Keystone Species - The 8th ICSAE<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/2021.icsae.id\/icsae8-video-id148\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"ICSAE8 Video ID148 Ficus septica, an Ecosystem Keystone Species - The 8th ICSAE\" \/>\n<meta property=\"og:description\" content=\"Paper ID: 148 Ficus septica, an Ecosystem Keystone Species Induced ROS-Mediated Cytotoxicity in HepG2 Hepatocarcinoma Cells S Haryanti*, N Rahmawati, I Y M Sholikhah and Y Widiyastuti Author Information Medicinal Plant and Traditional Medicine Research and Development Center, Ministry of Health, Jl. 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