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Paper ID: 7

Characterization of Acid Soluble Type I Collagen from Decapterus macarellus Skin

E Herawati1*, Y Akhsanitaqwim1, H Kristin1, V A Setyawan1 and A Ratriyanto2

1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret

2Department of Animal Husbandry, Faculty of Agriculture, Universitas Sebelas Maret


The growing demand on marine-based collagen is attributed to its high absorption rate and bioavailability. This study aimed at; 1) obtaining acid-soluble collagen extract from Decapterus macarellus skin; 2) characterizing the collagen extracted from D. macarellus. The skin was extracted using 0,5 M acetic acid, dialyzed, and freeze-dried to obtain sponge collagen. Molecular weight and characteristic of amide bands were evaluated using SDS PAGE and FTIR spectroscopy, respectively. The skin derived acid soluble collagen yielded 4,02%, physically white in color and odorless. Scanning electron micrographs revealed the fibrillar architecture of collagen that appears ‘flaky’ at higher magnification. SDS PAGE confirmed the presence of α1 (~ 120kDa), α2 (~ 100kDa), and β (~ 200kDa) chains, suggesting a typical of type I collagen as commonly found in the skin. FTIR spectra showed the presence of five typical functional groups of collagens, namely Amide A (3427.65 cm-1), Amide B (2940.61 cm-1), Amide I (1648.24 cm-1), Amide II (1532.51 cm-1) and Amide III (1238.25 cm-1). The present study demonstrates for the first time, extraction, and characterization of type I collagen from D. macarellus skin. Being one of the most abundant fish in tropical marine area, D. macarellus serves as a great resource for economical collagen-based biomaterials.

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