Paper ID: 49
Study on Genetic Variation of Kelembak (Rheum officinale Baill.) Using RAPD Molecular Markers
D Subositi*, N H Listyana and Widyantoro
Medicinal Plant and Traditional Medicine Research and Development Center, NIHRD-MoH, Jl Lawu no 11 Tawangmangu, Karanganyar, Central Java, Indonesia, 57792
Kelembak (Rheum officinale Baill.) is native to mainland Central China and reported as an endangered medicinal plant. This plant in Indonesia distributed in mountainous areas; the center of rhubarb cultivation is in the Dieng Plateau. Mainly this plant is grown for the roots as herbal medicine material (Kelembak Jamu) and in the industry for cigarette ingredients (kelembak Jawa). The objective of this study was to assess the genetic diversity of 9 accessions of R. officinale. Six selected RAPD primers were used to amplify the samples and produced 33 DNA fragments ranging from 270-2.710 bp. The Genetic similarity was ranged from 74.58% to 100% using Dice similarity index. The dendrogram divided R. officinale into 2 main clusters separated between kelembak Jamu and kelembak Jawa. Cluster analysis revealed that Mangli Accession (Kelembak jamu) had a closer similarity to Kelembak Jawa. The narrow genetic diversity of Kelembak is important information for future conservation and management programs in the cultivation of this species.