¹Student of Agrotechnology Department, Faculty of Agriculture, Sebelas Maret University, Surakarta, Indonesia

²Lecturer of Agrotechnology Department, Faculty of Agriculture, Sebelas Maret University, Surakarta, Indonesia

Email: nandar.suroso@yahoo.com

Gamma-ray irradiation at Cempo Ireng can lead to the variation of morphological characteristics so that morphological characterization is necessary to determine changes in potential lines. The aims of this research were to get descriptions of potential lines as one of the requirements for proposing plant variety release and add black rice genetic diversity information. The research was carried out in the rice fields of Pakahan Village, Jogonalan, Klaten in January-June 2020 with the arrangement of potential lines 8.13 and 44 of M6 in a row. 30 plants were selected randomly for each potential line and observed 19 morphological characters. Data analysis was carried out descriptively and qualitatively with the Chi-Square test. The results showed that gamma-ray irradiation affects the characters of leaf angle, auricle color, ligule color, leaf color, leaf surface, panicle type, grain color, apiculus color, and rice length. Potential line 8 had different characteristics compared to non-irradiated Cempo Ireng in the characters of auricle color, leaf blade color, leaf width, panicle length, grain width, rice length, and rice width, while on potential line 13 and 44 occur in the characters of leaf surface and panicle length.