¹Post Graduate Departement of Agronomy, Faculty of Agriculture, Sebelas Maret University, Surakarta, Indonesia

²Medicinal Plants and Traditional Medicines Research and Development Center (B2P2TOOT) Tawangmangu, Indonesia

³Departement of Agrotechnology, Faculty of Agriculture, Sebelas Maret University, Surakarta, Indonesia

Email: yunus.uns7@yahoo.com

Purple coneflower (Echinacea purpurea (L.) Moench) is classified as medicinal plant comes from North America and have been much developed in Indonesia. E. purpurea can be developed by utilizing suboptimal land such as saline land. This study aims to determine the effect of CaCl₂ concentration on growth and proline accumulation of three accessions E. purpurea. This study used a Completely Randomized Design (CRD) with 2 factors and five replications. The first factor is three accessions E. purpurea (accession 1; 2; and 3). The two factor is four concentrations CaCl₂ (0; 2.500; 5.000; and 10.000 ppm). The observations are plant height, number of leaves, number of branches and proline accumulation. Data were analyzed using SPSS to test difference. The results showed that highest concentration of CaCl₂ can inhibit growth and increase proline accumulation. The highest proline accumulation in accession 2 was 22,8002 µmol g^-1, accession 2 as an indicator of accession tolerant to salinity