Master Study of Soil Science, Graduate School, Universitas Sebelas Maret, Surakarta 57126, Indonesia

Email : retnobs@staff.uns.ac.id

Environmental pollution by heavy metals, especially Mercury (Hg), is one of the most serious environmental problems. The presence of excessive Mercury in the soil will cause soil degradation and threaten the life of the ecosystem in the soil, therefore remediation is necessary. Biduri is known to be able to absorb heavy metals, but there is no research on the ability of biduri to absorb Mercury. The use of bacteria, especially indigenous bacteria, is expected to increase the absorption of mercury by Biduri. Indigenous bacteria have a higher level of effectiveness in remediating soil contaminated with heavy metals. This indigenous bacteria was isolated from community gold mining in Jendi Village, Wonogiri. The purpose of the study was to determine the potential of biduri combined with indigenous bacteria and Agrobacterium sp I37 in absorbing mercury in the soil. The experimental design was factorial with a completely randomized design as the basic design, consisting of 2 factors: Bioremediation agent (A0: without bioremediation agent, A1: with indigenous bacteria, A3: Agrobacterium sp I37) and Mercury Dosage (D0: without Mercury, D1: Mercury 0.3 µg.g-¹, D2: Mercury 0.51 µg.g-¹.The data were analyzed by statistical analysis using ANOVA 95% significance level followed by Duncan Multiple Range Test 95% significance level, and correlation test. The results showed that the combination of Biduri with bacteria can increase the absorption of mercury by biduri, but indigenous bacteria with a dose of mercury 0.3mg/kg (A1D1) has the highest absorption of Hg 57.19 µg (27 times higher than control) biduri plants are able to translocate mercury from roots to shoots, and was able to reduce soil Hg levels by 0.09 µg.g-¹ or 30.76%  higher than the initial soil Hg content. Treatment of AID1 has a bioremediation effectiveness value of 79.07%, or 2 times less higher than the control. Bioremediation using indigenous bacteria was able to increase the total absorption compared to the control. Biduri as hyperaccumulator is 57.19 µg (higher than 10 µg.g-¹)