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Paper ID: 107

Genetic Diversity based on RAPD F2 Generation of Apple Cactus (Cereus spp.)

D E Anindyaswari1, Sukaya2*, E S Muliawati2 and D W Djoar2

1Undergraduate Program of Agrotechnology, Faculty of Agriculture, Sebelas Maret University

2Department of Agrotechnology, Faculty of Agriculture, Sebelas Maret University


Apple cactus which consists of 2 types, long spines (Cerues jamacaru) and short spines (C. peruvianus) both are self-incompatible. So that only artificial crosses between species that produce fruit. But are found some fruit from natural crosses on the type of long spines. The three results of these crosses, long spines with short spines; short spines with long spines and natural crosses on long spines have produced F2 seedlings. The purpose of this study was to study genetic diversity, based on the RAPD (Random Amplified Polymorphic DNA) of the F2 seedlings. DNA isolation was carried out using Doyle and Doyle (1990) method modified on the addition of 0.045 gr Polivinilpirolidon. DNA extraction by the Doyle and Doyle method. The RAPD technique uses 3 primers: OPD-11, OPM-10 and OPN-5. Analysis of genetic diversity/similarity using Simple Mathching coefficient using NTSys 2.02 software. Based on the three primers, each F2 offspring had genetic diversity, but grouped according to the parent. Showed that the three RAPD primers were effective for markers of genetic diversity in apple cactus.

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